Researchers at Adimab, LLC isolated 188 monoclonal antibodies against Crimean-Congo hemorrhagic fever virus (CCHFV) GP38 from human survivors. While non-neutralizing, these antibodies target 11 overlapping sites and demonstrate protective efficacy in rodent models. This study provides valuable insights into CCHFV viral glycoprotein and could aid the development of antibody therapeutics for this lethal virus.

Researchers at the University of Delaware used the INFORS HT Multitron incubator shaker to evaluate biolayer interferometry (BLI) for fast, high-throughput AAV capsid titer measurement. The study highlights the accuracy of AAVX-BLI across multiple rAAV serotypes, making it a strong alternative to ELISA. Applied in transfection optimization and AAV purification, this method offers valuable insights for improving rAAV production processes.

Gene therapy is a promising approach for treating various genetic disorders and diseases. A critical component of gene therapy is the production of viral vectors, which serve as delivery vehicles for therapeutic genes. Human Embryonic Kidney 293 (HEK293) cells have become a widely used platform for viral vector production due to their efficiency in transfection and ability to support viral replication. However, optimizing HEK293 cell cultures for large-scale production of viral vectors remains a challenge in making gene therapies more accessible and cost-effective.

Simple actions can make a lot of difference to the outcome of your shake flasks cultures. Some beneficial ones have been covered in previous articles, however, common practices can also be a barrier to effective optimization. This article will explain why these common practices count as sins against your shake flask culture and what you can do to overcome them.